Malondialdehyde(MDA)isthefinalproductoflipidperoxidation,andMDAcontentcanreflectthestresstoleranceofplants.TomapQTLsconditioningtheMDAcontentinriceleaves,arebinantinbredline(RIL)populationwith247linesderivedfromanindica-indicacrossZhenshan97B′Milyang46,andalinkagemapconsistingof207DNAmarkerswereused.TheRILpopulationshowedatransgressivesegregationintheMDAcontentofriceleaves.TwoQTLsfortheMDAcontentinriceleavesweredetectedintheintervalsRG532-RG811andRG381-RG236onchromosome1,withtheadditiveeffectsfrommaternalandpaternalparents,accountingfor4.33%and4.62%ofphenotypevariations,respectively.

ZHUANGJie-yun,FANYe-yang(ChineseNationalCenterforRiceImprovement/StateKeyLaboratoryofRiceBiology,ChinaNationalRiceResearchInstitute,Hangzhou310006,China)?

第2篇：Identification and genetic mapping of four novel genes that regulate leaf development in Arabidopsis

MolecularandgeiccharacterizationsofmutantshaveledtoabetterunderstandingofmanydevelopmentalprocessesinthemodelsystemArabidopsisthaliana.However,theleafdevelopmentthatisspecifictoplantshasbeenlittlestudied.Withtheaimofcontributingtothegeicdissectionofleafdevelopment,wehaveperformedalarge-scarescreeningformutantswithabnormalleaves.AmongagreatnumberofleafmutantswehavegeneratedbyT-DNAandtransposontaggingandethylmethaesulfonate(EMS)mutagenesis,fourindependentmutantlineshavebeenidentifiedandstudiedgeically.PhenotypesofthesemutantlinesrepresentthedefectsoffournovelnucleargenesdesignatedLL1(LOTUSLEAF1),LL2(LOTUSLEAF2),URO(UPRIGHTROSETTE),andEIL(ENVIRONMENTCONDITIONINDUCEDLESION).Thephenotypicanalysisindicatesthatthesegenesplayimportantrolesduringleafdevelopment.Forthefurthergeicanalysisofthesegenesandthemap-basedcloningofLL1andLL2,wehavemappedthesegenestochromosomeregionswithanefficientandrapidmappingmethod.

上海水磨工作室HAlHUANG(ShanghaiInstituteofPlantPhysiology,ChineseAcademyofScience,300FenglinRoad,Shanghai200032,ChinaShanghaiResearchCenterofLifeSciences,ChineseAcademyofSciences,320YueyangRoad,Shanghai200031,China)

YINGLIGUO(SchoolofLifeSciences,UniversityofScienceandTechnologyofChina.P.O.Box4,Hefei,Anhui230027,Chin)?

第3篇：OsDREB1 Gene from Rice Enhances Cold Tolerance in Tobacco

TheOsDREB1genefromriceencodesatranscriptionfactorbelongingtotheDREBPtranscriptionfactorsubfamily.ManyDREBPtranscriptionfactorsregulategeneexpressioninresponsetodrought,high-salt,andcoldstressesbybindingspecificallytothedehydration-responsiveelement(DRE).DRE-bindingproteins,suchasCBF1,DREB1A,andDREB2A,havebeenclonedfromArabidopsisthalianaandhavebeenprovedtoplayanimportantroleinstressresponseofArabidopsisandseveralotherplants.Inthisstudy,theOsDREB1genewastransferredtotobaccoplantsbytheAgrobacterium-mediatedtransfermethod,and16transgenicplantswereidentified.PCRanalysisdemonstratesthattheforeigngeneshavebeenintegratedintothetobaccogenome.Resultsoffreezingstressexperimentsindicatethatthetransgenicplantshaveenhancedcoldtolerance.